Journal: Journal of Molecular Histology
Article Title: Autophagy hub genes mediate photodynamic therapy tolerance in nasopharyngeal carcinoma through cytoprotective autophagy and survival signaling
doi: 10.1007/s10735-026-10746-x
Figure Lengend Snippet: PDT induces autophagic flux in nasopharyngeal carcinoma cells. A This panel shows representative western blots of LC3-II, p62/SQSTM1, and GAPDH in C666-1 and HONE1 cells, respectively, treated with PDT alone or in combination with rapamycin (autophagy activation) or chloroquine (autophagy inhibition). B This panel shows densitometric quantification of LC3-II and p62 normalized to GAPDH, respectively, presented as mean ± SD from independent experiments performed separately in each cell line. P ***-value < 0.001
Article Snippet: Membranes were blocked in 5% non-fat milk in TBST and incubated overnight at 4 °C with primary antibodies against AKT1 (CST #2938, 1:1000), BECN1/Beclin-1 (CST #3738, 1:1000), BCL2 (CST #2876, 1:1000), ATG5 (CST #2630, 1:1000), LC3B (CST #3868, 1:1000), p62/SQSTM1 (CST #5114, 1:1000), and the loading control GAPDH (CST #2118, 1:5000).
Techniques: Western Blot, Activation Assay, Inhibition