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loading control  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc loading control
    Loading Control, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 10892 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loading control/product/Cell Signaling Technology Inc
    Average 99 stars, based on 10892 article reviews
    loading control - by Bioz Stars, 2026-05
    99/100 stars

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    PDT induces autophagic flux in nasopharyngeal carcinoma cells. A This panel shows representative western blots of <t>LC3-II,</t> <t>p62/SQSTM1,</t> and <t>GAPDH</t> in C666-1 and HONE1 cells, respectively, treated with PDT alone or in combination with rapamycin (autophagy activation) or chloroquine (autophagy inhibition). B This panel shows densitometric quantification of LC3-II and p62 normalized to GAPDH, respectively, presented as mean ± SD from independent experiments performed separately in each cell line. P ***-value < 0.001
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    Image Search Results


    Relative mRNA expression levels of CALR, SMC4, P53, MCM10 and SRCIN1, which were validated by Q-PCR analysis normalized to GAPDH. The data are expressed as mean ± SD. **: p < 0.01 (the androgen-activated AP vs. the androgen-unactivated AP, n = 3).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: The regulation of pedicle initiation by androgens in sika deer (Cervus nippon)

    doi: 10.3389/fcell.2025.1708732

    Figure Lengend Snippet: Relative mRNA expression levels of CALR, SMC4, P53, MCM10 and SRCIN1, which were validated by Q-PCR analysis normalized to GAPDH. The data are expressed as mean ± SD. **: p < 0.01 (the androgen-activated AP vs. the androgen-unactivated AP, n = 3).

    Article Snippet: Antibodies used in the Western blot analysis included: anti-GAPDH-loading control monoclonal antibody (bsm-33033M, Bioss, China), anti-SRCIN1 antibody (D153560, BBI life sciences, China), anti-Calreticulin polyclonal antibody (bs-5913R, Bioss, China), anti-SMC4 polyclonal antibody (bs-7728R, Bioss, China), anti-p53 protein (wt-p53) polyclonal antibody (bs-0033R, Bioss, China).

    Techniques: Expressing

    PDT induces autophagic flux in nasopharyngeal carcinoma cells. A This panel shows representative western blots of LC3-II, p62/SQSTM1, and GAPDH in C666-1 and HONE1 cells, respectively, treated with PDT alone or in combination with rapamycin (autophagy activation) or chloroquine (autophagy inhibition). B This panel shows densitometric quantification of LC3-II and p62 normalized to GAPDH, respectively, presented as mean ± SD from independent experiments performed separately in each cell line. P ***-value < 0.001

    Journal: Journal of Molecular Histology

    Article Title: Autophagy hub genes mediate photodynamic therapy tolerance in nasopharyngeal carcinoma through cytoprotective autophagy and survival signaling

    doi: 10.1007/s10735-026-10746-x

    Figure Lengend Snippet: PDT induces autophagic flux in nasopharyngeal carcinoma cells. A This panel shows representative western blots of LC3-II, p62/SQSTM1, and GAPDH in C666-1 and HONE1 cells, respectively, treated with PDT alone or in combination with rapamycin (autophagy activation) or chloroquine (autophagy inhibition). B This panel shows densitometric quantification of LC3-II and p62 normalized to GAPDH, respectively, presented as mean ± SD from independent experiments performed separately in each cell line. P ***-value < 0.001

    Article Snippet: Membranes were blocked in 5% non-fat milk in TBST and incubated overnight at 4 °C with primary antibodies against AKT1 (CST #2938, 1:1000), BECN1/Beclin-1 (CST #3738, 1:1000), BCL2 (CST #2876, 1:1000), ATG5 (CST #2630, 1:1000), LC3B (CST #3868, 1:1000), p62/SQSTM1 (CST #5114, 1:1000), and the loading control GAPDH (CST #2118, 1:5000).

    Techniques: Western Blot, Activation Assay, Inhibition